Proteins Of Bovine Red Blood Cell - 1376 Words

Protein Composition in Red Blood Cells in Humans using Polyacrylamide Gel Electrophoresis Name: Emma Claypole Date: Wednesday March 16, 2016 Lab Group: W08, Wednesday morning 2 Abstract The proteins of Bovine red blood cell (RBC) membranes were analyzed using polyacrylamide gel electrophoresis. After analyzing Bovine RBC they were then compared to human RBC counterpart. Following finding the log of each molecular weight of each band, band one showed the highest molecular weight. All five bands viewed were from humans. There are typically 7 bands visible however, in our case only five were visible due to implications within the gel sample. Introduction Proteins are important elements in cellular membranes and give the membranes many of their characteristics. In red blood cells, the meshwork of proteins in and around the membrane gives it strength and flexibility, allowing a cell to squeeze through small capillaries without bursting. Other proteins play roles in transporting material in and out of the cell (Lab Manual, Cell Biology). Polyacrylamide gel electrophoresis (PAGE), with all of its different modifications is probably the most widely-utilized procedure in contemporary biochemistry and molecular biology (Mordacq and Ellington (1994)). In this experiment, we will attempt to determine the molecular weights of the major proteins in the plasma membranes of bovine red blood cells (RBCs). The predictions made are if our protein has similar weights as proteinsShow MoreRelatedRecurrent Incidence Of Babesiosis Among Adults Essay2009 Words   |  9 PagesRecurrent incidence of babesiosis among apparently healthy bovines in Egypt, sub mits the needs for suitably destined prevalence studies with rapid and accurate diagnosis and immediate treatment of infected animals are required to control this disease. 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The Life of the American Born Free Essays

This paper examines the life of the American born, Tim O’Brien and affirms the way his life influenced his writing techniques. William Timothy, the first of three children, well known as Tim O’Brien is an American Veteran writer, born on October 1, 1946, in Austin, Minnesota, United States of America (Herzog 14). He grew up in Worthington, a small shielded community near the borders of Iowa and South Dakota. We will write a custom essay sample on The Life of the American Born or any similar topic only for you Order Now He described himself as a vivid reader when he was young and had a thrilling interest in books and magic trick were evident realities on his storytelling power. This can also be linked to his parents who were enthusiastic readers. In other words, O’Brien must have learned some reading secrets from his parents when he was young. Some of his short and electrifying stories include, â€Å"The things they carried† as well as other classic novels like the war novel, going after Cacciato produced in1978, which indeed made him known best for his writing experience about Vietnam War (Raymond 102). Worthington town similarly played a significant role in nurturing his thoughts while developing the artistic sensibility of his life as an author. He attended Macalester College in 1968 and awarded bachelor’s degree in Political Science. During his time in the institution, he was elected and served as the president of the student’s union in college. Contrary to his criticisms against war, Tim O’Brien was recruited into United States Army and then sent to Vietnam where he served for two years. Following his return from the military service, he resumed his studies and graduated from Harvard University. He then worked shortly as an intern, national affairs reporter at Washington Post up to May 1974. The war experience he gained from his two-year combat inspired his chief publication, titled If I Die in a Combat Zone, Box Me up and Ship Me Home. O’Brien’s history clearly reveals the depression and misery wrecked on soldiers by the Vietnam War. His mythical work, the things they curried of 1990, (O’Brien 816) is a combination of memoirs and fictitious stories in which he used the philosophical concepts of truth to climax the distinctions between two realms of the facts and the fictions in a unique way (Conan 2010). He incorporated the metaphysical approach attribute in his writing that flavored the quality of his stories. In his story, he exposes the readers to the understanding about the distinction between the truth of a story, the truth of fiction and the happening truth, which is associated with the truth of facts. In confirming the same, he appreciates that a story truth is more associated with emotion, which are also connected with the feelings of the story of fiction. On that pedestal, he adds that the feelings would be more significant, and thus making the story more relevant. Additionally, O’Brien wrote numerous novels based on the war experience he obtained in the army of the United States. He was awarded the 1979 National Book Award for his works on Northern Lights published in1975 and Going after Cacciato of 1978. The novel discusses two brothers against one and another as foils where a brother joined the Vietnam war andanother failed (Raymond 99). Through this, the brothers learned to struggle and comprehend each one’s values and motives. Following his extraordinary influence of Joseph Conrad’s works, he modified Conrad’s concepts of human aptitude for good and evil in his literary works. In his works, O’Brien propounded timeless matter of the subject and its thematic range. He believed in his introversive idea, that literary works not only focus on the current affairs but also anticipates what the future holds for humankind, (Smith 34). Nuclear Age, which was published in 1985 was the third O’Brien’s novel of his literary works and describes much from his personal experience. His expertise in creating an amusing look at the thoughtful character, a young man who was raised under an atomic umbrella, and suffered severely from paranoia as a result of nuclear war. As the story continues, he finds solace in a hole he dug in his backyard attempting to overcome every thought and imagination, which made him uncomfortable. His creativity in writing approach of war blending fact and fiction is comparable to other technical war writers like Michael Herr and inspired eminent figures such as Truman Capote, Norman Mailer and Tom Wolfe. However, key elements of his works encompass the categorization and fascination of emotional subject matters. Owing to this traits, his fictional works are therefore considered to have made an immense impact on the readers of his novels. Other O’Brien’s electrifying works are The Nuclear Age, published in 1985, In the Lakes of The Woods of 1994, Tomcat in Love of 1998 and July, July that was published in 2000, which took the world of literature with a storm. In a span of two years, O’Brien’s short stories The things they curried, received the 1987 National Magazine Award Fiction. The novel was then nominated for 1987, best American Short story. The lake of Woods forms another interesting novel written by O’Brien and published in 1994. It took O’Brien’s works into greater heights by agitating much on guilt, culpability, complicity and the moral courage, (Bates 265). He invents his character John Wade, who is also a Vietnam veteran, aspiring to run for senate position. He later lost slightly as charges on his involvement in the Lai massacre became popular during his campaign trail. O’Brien raises larger inquiries about the fallouts of war and its effects, which was realized after the end of the war, more so when the veterans returned home. He wore James Fenimire Cooper Prize from the Society of American Historians and was selected as the best novel of 1994 by Times Magazine.His most recent Novel, Tomcat in Love, O’Brien generated a Veteran Vietnam protagonist, Tom chippering through a love subject contrary to war. O’Brien describes this as a comic novel about a politically incorrect hero, who was sexy. He strongly believed that his lovers will love the book, and his fans, the so-called junkies, the people who appreciate his writings liked it. He alludes that this was his best book ever and it stole the hearts of many who could not do without reading the novel.In conclusion, O’Brien has won several prestigious awards in his many fictional works and worth being called a veteran in the writing field. His experience in writing the short story novels are highly acknowledged. He is a master of creative storytelling, a manipulator of literary form and one of the most challenging authors in his time with an ample content. His major contributions include the Nuclear Age, In the Lake of Woods, Tomcat in Love and July, July of 20000 More recently, he received $100,000 with the Pritxker Military Library Literature Award. Currently, he teaches at the Texas State University-San Marcos. How to cite The Life of the American Born, Papers

Anthony, soaked in his own perspiration after a friendly soccer match, barged through his classroom door angrily Essay Example For Students

Anthony, soaked in his own perspiration after a friendly soccer match, barged through his classroom door angrily Essay Anthony, soaked in his own perspiration after a friendly soccer match, barged through his classroom door angrily, with his soccer ball within his arm. He sat down, disposing his gear- grassy socks with its stink, goalkeepers gloves à ¢Ã¢â€š ¬Ã¢â‚¬Å" into an NTUC Fairprice plastic bag. The thirteen-year-old then lay back in his chair, deep in thoughts, unaware of the quietness of an empty classroom late in the afternoon. Boy, what an idiot you are! If you do not turn up for the next soccer practice, Ill replace you with that boy from 1C, that Weixiang! à ¢Ã¢â€š ¬Ã¢â‚¬Å" The sight of his soccer match, Mr. Anderson, yelling into his face kept flashing within Anthonys mind. The pot-bellied English man had a reputation for deafening his students with his voice, not much difference from using a loud hailer. Anthony is proud of his position, as goalkeeper of the C Division soccer team of the school. Never could he imagine Weixiang, that scrawny egg-shaped spectacles-rimmed nerdy homo-sapien replacing that post due to missing a few soccer practices for the sake of competing at Counterstrike games with his long-timed rivals at the cybercafe nearby. It is a hard-earned achievement for Anthony Tan, previous top-scorer in his lowly unheard Primary School; he has intentions of converting from his library-dwelling old self, to the sporty, sociable stud of Bukit Timah Secondary School. The stud packed his school bag. His eyes felt dry. Darn. I have been wearing these contact lenses since six this morning, he thought, rubbing his left eye with the back of his hand. All he wanted to do was to shoot his way home to remove the irritating focuses. Anthony swung the bag across his back in a fashionable manner à ¢Ã¢â€š ¬Ã¢â‚¬Å" similar to those American kids on TV hanging out at their school lockers. He dribbled his favorite ball à ¢Ã¢â€š ¬Ã¢â‚¬Å" most desired 13th birthday gift, indication of start to teenage-hood. Out of the dark classroom, along the dimly lit corridors, attempting to make his way out of the school compound while showing himself as a well-inspired fan of Paolo Maldini. Dribbling on, with pillars in place as his defenders. He stopped. Slowly, in a dramatic manner, our hero ascended his right leg to a certain level. Anthony gave the soccer ball a hard deserving kick. How he wished it was the head of his Mathematics teacher. Our friend then discovered à ¢Ã¢â€š ¬Ã¢â‚¬Å" venging anger on non-living objects with the hint of cruel imagination does cure vengeance within the heart. He was unaware of where the launched head went, until the deafening sound of something breaking. Oh no, Anthony grumbled. He found his way in front of the schools display gallery, where they had the works from the art enthusiasts of the school to show off. Anthony stared at his soccer ball, currently lying beside one of the bougainvillea flowerpots that were meant to beautify the entrance of the gallery. The clay pot, holding the plant root upright, was in several pieces. Soil was spilled all over the floor. The devil arrived at the scene. Running his fingers through the silkiness of his hair, Brandon Lee looked like a teenage model for shampoo advertisement. He is, too, the soccer teams star attacker who makes pubescent girls swoon at him at his knees. His reputation has earned him popularity and an overworked ego. Hey goalkeeper! he taunted. Thats a good shot! Brandon, in his fresh clothes and smell of expensive cologne, bent over and helped picked up the pieces of clay slowly. .u7bd979b091e87badef054e984339540e , .u7bd979b091e87badef054e984339540e .postImageUrl , .u7bd979b091e87badef054e984339540e .centered-text-area { min-height: 80px; position: relative; } .u7bd979b091e87badef054e984339540e , .u7bd979b091e87badef054e984339540e:hover , .u7bd979b091e87badef054e984339540e:visited , .u7bd979b091e87badef054e984339540e:active { border:0!important; } .u7bd979b091e87badef054e984339540e .clearfix:after { content: ""; display: table; clear: both; } .u7bd979b091e87badef054e984339540e { display: block; transition: background-color 250ms; webkit-transition: background-color 250ms; width: 100%; opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #95A5A6; } .u7bd979b091e87badef054e984339540e:active , .u7bd979b091e87badef054e984339540e:hover { opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #2C3E50; } .u7bd979b091e87badef054e984339540e .centered-text-area { width: 100%; position: relative ; } .u7bd979b091e87badef054e984339540e .ctaText { border-bottom: 0 solid #fff; color: #2980B9; font-size: 16px; font-weight: bold; margin: 0; padding: 0; text-decoration: underline; } .u7bd979b091e87badef054e984339540e .postTitle { color: #FFFFFF; font-size: 16px; font-weight: 600; margin: 0; padding: 0; width: 100%; } .u7bd979b091e87badef054e984339540e .ctaButton { background-color: #7F8C8D!important; color: #2980B9; border: none; border-radius: 3px; box-shadow: none; font-size: 14px; font-weight: bold; line-height: 26px; moz-border-radius: 3px; text-align: center; text-decoration: none; text-shadow: none; width: 80px; min-height: 80px; background: url(https://artscolumbia.org/wp-content/plugins/intelly-related-posts/assets/images/simple-arrow.png)no-repeat; position: absolute; right: 0; top: 0; } .u7bd979b091e87badef054e984339540e:hover .ctaButton { background-color: #34495E!important; } .u7bd979b091e87badef054e984339540e .centered-text { display: table; height: 80px; padding-left : 18px; top: 0; } .u7bd979b091e87badef054e984339540e .u7bd979b091e87badef054e984339540e-content { display: table-cell; margin: 0; padding: 0; padding-right: 108px; position: relative; vertical-align: middle; width: 100%; } .u7bd979b091e87badef054e984339540e:after { content: ""; display: block; clear: both; } READ: A Wrinkle In Time EssayAnthony joined him, rather grateful. Yet the other stopped his kind action, standing up to leave. Right, I should not be doing this! he laughed. You better clear up this mess quick. Perhaps the school authorities will overlook the plant, Brandon? Will you keep mum about this? Anthony was practically begging. It depends, Brandon grinned virulently, turning to leave the quiet school compounds. Anthony could sense something was not right, yet he had more things to worry about, continuing to clear up the mayhem of spilled soil and broken clay as the evening gloom filled the school compound. Back at home, Anthonys hands were cleaned from all that soil. He sat at his study table, struggling with Shakespeares language in his text of Julius Caesar. The irritating loud ring of his telephone sounded. Anthony jumped from his seat, rushing to pick it up, with high hopes that it was Maria, his current infatuation. Hello? he said calmly. Anthony, please, the voice on the other end was a male. Speaking, his hopes fell. This is Brandon here, goalie! Brandon? He must be up to no good What is it? Anthony asked. Brandon wanted Anthony to help him to do a copy of literature assignment. You know, boy, he said in a mocking-accent, I am too tired off after that soccer match, after scoring so many goals, But I havent done the same assignment myself, Anthony tried. The flower pot? It was amazing how the mention of that certain object could trigger fear within our friend! Anthony knew that Brandon was up to no good! Okay, okay, Ill do it! he obliged reluctantly. Placing down the handset, Anthony punched the wall indignantly. Threatening things could get, it seems. He recalled getting bullied in Primary school, yet Brandon could not be considered a bully. All those threats from his little school mates, jeering I dont want to friend you. Childishness. Thinking that a change in school, growing to be a teenager, would meant a change from all that childishness, and threats. A stronger will to reject. Brandons threat begged to differ from Anthonys new perspective. How bad life is. Perhaps the saying of having only two kinds of people-One a leader, one a follower, seems astoundingly true.

Reflection Analysis Observations

Introduction Group dynamic is essential in determining its course. Our group was characterized with trust, care, and fairness. In fact, no one cared to note each other’s background. We accepted one another as equals within the group. This was essential in enabling us to achieve group cohesion. Moreover, it aided us in performing group activities without hindrance. This paper will reflect on leadership, group theory, and group skills (D.W. Johnson P.J. Johnson, 2013, p. 27).Advertising We will write a custom essay sample on Reflection Analysis Observations specifically for you for only $16.05 $11/page Learn More Theme, dynamic or pattern noticed Our members were democratic, trustworthy, and caring. These important elements were achieved early in the group meetings through sharing of roles within the group (Johnson, D.W., Johnson, 2013, p.121). In fact, even though one of us had more experience with aircraft than the rest, she never showed it in a manner to suggest intimidation. We considered ourselves equal and in each turn, a new DA was selected to moderate our group tasks. The group was therefore democratic and showed a pattern of trust and leadership (D.W. Johnson P.J. Johnson, 2013, p. 122). Provisional explanation/hypothesis The group did not have age and careers issues. In fact, as said earlier, only one person was knowledgeable on aircraft issues. We therefore depended heavily on her solutions for a single session only. Moreover, the attitude in the group did not allow us to consider aspects like age and careers as issues of concern. Instead, we treated each other equally. In essence, everyone had equal opportunities to be the DA. Evidence The fact that everyone got equal chances of becoming a DA as well as in contributing to the solution of issues in the group did not allow us to classify ourselves in any category, class, or age. In fact, as said earlier, only one individual had experience in aircraft related task. This was only helpful in the second discussion. However, she still ensured that our discussions were taken into consideration after an excerpt from her experience. Analysis of theme, dynamic or pattern The dynamic, pattern or theme of our group revealed both formal and informal authority. However, this depended on the kind of role given to each individual after a democratic selection. For instance, the Designated Authority (DA) moderated group activities. This afforded him/her formal authority over us during group proceedings. However, other tasks like note taking, observers, among others toke informal authority as they took keen interest on contributions from each individual.  However, it is necessary to not that the first meeting was quite cautious and everyone, including myself, was quite hesitant to take formal authority. Fortunately, one of the group members volunteered and we never looked back (D.W. Johnson P.J. Johnson, 2013, p. 122).Advertising Looking for essay on education? Let's see if we can help you! Get your first paper with 15% OFF Learn More Discomforting evidence that exists The group did not have any issues. In fact, only the first meeting was characterized with high tensions. However, this was because we had not met as a group before. Moreover, we did not know what was required of us in the group. In addition, we were hesitant to give contributions. However, this did not affect us anymore after the first meeting. In fact, we gelled quite easily and we were able to agree to most solutions in our discussions. Conclusion Our group was characterized with leadership, trust, and care. In fact, everyone was involved in-group discussions. Except for the first meeting, everyone was happy to contribute to the discussions. Moreover, we gelled quite easily since cohesion was achieved quickly within the group. In addition, we trusted each other quite easily and decided on issues through democratic ways. However, we also gave the minorit y and opportunity to explain reasons for their choices. Group dynamic was therefore achieved with leadership skills observable in each individual (D.W. Johnson P.J. Johnson, 2013, p. 122). Reference Johnson, D.W., Johnson, P.J. (2013). Joining Together: Group Theory and Group Skills (11th ed.). Upper Saddle River, NJ: Pearson Education, Inc. This essay on Reflection Analysis Observations was written and submitted by user Benson L. to help you with your own studies. You are free to use it for research and reference purposes in order to write your own paper; however, you must cite it accordingly. You can donate your paper here.